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Image Search Results
Journal: Cell transplantation
Article Title: Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an In Vitro Model of Osteoarthritis.
doi: 10.1177/0963689718804130
Figure Lengend Snippet: Fig. 1. Experimental scheme and L1000 array analysis. (A) Seeded SW1353 cells were serum-starved under hypoxic conditions for 12 hours and then stimulated with 10 ng/ml of IL-1b or PBS for 24 hours followed by treatment with 100 mg/ml of PDRN. After 24 hours, cells were harvested for the L1000 array. To validate the L1000 array study and OA pathogenesis, qRT-PCR and a western blot were conducted. To examine the migration efficiency, an in vitro scratch assay was performed. (B) The number of enriched proteins is indicated by bar graphs. The number of up-regulated proteins is indicated by a red bar while, the number of down-regulated proteins is indicated by a green bar. IL: interleukin; OA: osteoarthritis; PBS: phosphate-buffered saline; PDRN: polydeoxyribonucleotide; qRT-PCR: quantitative real-time polymerase chain reaction.
Article Snippet: A
Techniques: Quantitative RT-PCR, Western Blot, Migration, In Vitro, Wound Healing Assay, Saline, Real-time Polymerase Chain Reaction